Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency
Joint Authors
Sheridan, Steven D.
Surampudi, Vasudha
Rao, Raj R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-01
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Human induced pluripotent stem cells (hiPSCs) have core properties of unlimited self-renewal and differentiation potential and have emerged as exciting cell sources for applications in regenerative medicine, drug discovery, understanding of development, and disease etiology.
Key among numerous criteria to assess pluripotency includes the in vivo teratoma assay that has been widely proposed as a standard functional assay to demonstrate the pluripotency of hiPSCs.
Yet, the lack of reliability across methodologies, lack of definitive clinical significance, and associated expenses bring into question use of the teratoma assay as the “gold standard” for determining pluripotency.
We propose use of the in vitro embryoid body (EB) assay as an important alternative to the teratoma assay.
This paper summarizes the methodologies for creating EBs from hiPSCs and the subsequent analyses to assess pluripotency and proposes its use as a cost-effective, controlled, and reproducible approach that can easily be adopted to determine pluripotency of generated hiPSCs.
American Psychological Association (APA)
Sheridan, Steven D.& Surampudi, Vasudha& Rao, Raj R.. 2012. Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency. Stem Cells International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1003093
Modern Language Association (MLA)
Sheridan, Steven D.…[et al.]. Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency. Stem Cells International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1003093
American Medical Association (AMA)
Sheridan, Steven D.& Surampudi, Vasudha& Rao, Raj R.. Analysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotency. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1003093
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003093