Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture
Joint Authors
Jensen, Pia S.
Lyck, Lise
Jensen, Pia
Zimmer, Jens
Meyer, Morten
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-20
Country of Publication
Egypt
No. of Pages
13
Abstract EN
The potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like Parkinson’s disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons.
One current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-term in vitro propagation of the cells.
The domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resemblance of the larger gyrencephalic pig brain to the human brain than the commonly used brains of smaller rodents.
In the present study, porcine embryonic (28–30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2.
For passaging, the tissue spheres were cut into quarters, avoiding mechanical or enzymatic dissociation in order to minimize cellular trauma and preserve intercellular contacts.
Spheres were propagated for up to 237 days with analysis of cellular content and differentiation at various time points.
Our study provides the first demonstration that porcine ventral mesencephalic precursor cells can be long-term propagated as neural tissue spheres, thereby providing an experimental 3D in vitro model for studies of neural precursor cells, their niche, and differentiation capacity.
American Psychological Association (APA)
Jensen, Pia S.& Lyck, Lise& Jensen, Pia& Zimmer, Jens& Meyer, Morten. 2012. Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture. Stem Cells International،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1003100
Modern Language Association (MLA)
Jensen, Pia S.…[et al.]. Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture. Stem Cells International No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1003100
American Medical Association (AMA)
Jensen, Pia S.& Lyck, Lise& Jensen, Pia& Zimmer, Jens& Meyer, Morten. Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1003100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003100