Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells
Joint Authors
Nuzzi, Raffaele
Gunetti, Monica
Rustichelli, Deborah
Roagna, Barbara
Fronticelli Bardelli, Francesca
Fagioli, Franca
Ferrero, Ivana
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-07
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Age-related macular degeneration (AMD) is a leading cause of legal blindness in individuals over 60 years of age, characterized by the dysfunction of retinal pigmented epithelium cells, specifically in the macular area.
Despite several treatment options, AMD therapy remains difficult, especially for exudative AMD.
Multipotent mesenchymal stem cells (MSCs), with great plasticity and immunomodulant properties, are a promising cell source for cellular therapy and tissue engineering.
We evaluated the effects of steroid drugs, often used to treat AMD, in association with MSCs, in view of a possible application together to treat AMD.
Morphology, viability, growth kinetics, and immunophenotype were evaluated on healthy donors’ MSCs, treated with triamcinolone acetonide, alcohol-free triamcinolone acetonide, micronized intravitreal triamcinolone and dexamethasone at different concentrations, and in a human retinal pigment epithelial cell line supernatant (ARPE-19).
The morphological analysis of MSCs in their standard medium showed a negative correlation with drug concentrations, due to the numerous crystals.
Dexamethasone was the least toxic corticosteroid used in this study.
ARPE-19 seemed to help cells preserve the typical MSC morphology.
In conclusion, this in vitro study demonstrated that high doses of corticosteroid drugs have a negative effect on MSCs, reduced in the presence of a conditioned media.
American Psychological Association (APA)
Nuzzi, Raffaele& Gunetti, Monica& Rustichelli, Deborah& Roagna, Barbara& Fronticelli Bardelli, Francesca& Fagioli, Franca…[et al.]. 2012. Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells. Stem Cells International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1003133
Modern Language Association (MLA)
Nuzzi, Raffaele…[et al.]. Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells. Stem Cells International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1003133
American Medical Association (AMA)
Nuzzi, Raffaele& Gunetti, Monica& Rustichelli, Deborah& Roagna, Barbara& Fronticelli Bardelli, Francesca& Fagioli, Franca…[et al.]. Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1003133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003133