PHBVPCL Microparticles for Controlled Release of Resveratrol: Physicochemical Characterization, Antioxidant Potential, and Effect on Hemolysis of Human Erythrocytes

Abstract EN

Microparticles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) containing resveratrol were successfully prepared by simple emulsion/solvent evaporation.

All formulations showed suitable encapsulation efficiency values higher than 80%.

PHBV microparticles revealed spherical shape with rough surface and presence of pores.

PCL microparticles were spherically shaped with smooth surface.

Fourier-transformed infrared spectra demonstrated no chemical bond between resveratrol and polymers.

X-ray powder diffraction patterns and differential scanning calorimetry analyses indicated that microencapsulation led to drug amorphization.

These PHBV/PCL microparticles delayed the dissolution profile of resveratrol.

Release profiles were better fitted to biexponential equation.

The hypochlorous-acid-scavenging activity and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation discoloration assay confirmed that the antioxidant activity of PHBV/PCL microparticles was kept, but was dependent on the microparticle morphology and dissolution profile.

Resveratrol-loaded PHBV/PCL microparticles showed no cytotoxic effect on red blood cells.