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Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows
Joint Authors
Watanabe, Atsushi
Hirota, Jiro
Inumaru, Shigeki
Shimizu, Shinya
Kimura, Kazuhiro
Source
Veterinary Medicine International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot formation from 1 to 28 days post infusion (PI).
In the mammary secretions of the mastitic glands, high levels of IL-8 were sustained from 8 hours to 28 days PI, peaking at 1–3 days PI.
The levels of leukocyte-derived elastase and inflammatory 22 and 23 kDa lactoferrin derived peptides (LDP) were also increased in the mammary secretions from the mastitic glands.
In addition to the experimentally induced mastitis, the mammary secretions from the glands of cattle with spontaneous Staphylococcus aureus dry-period mastitis displayed milk clot formations and significant increases in their levels of PMNL counts, elastase, LDP, and IL-8, compared with those of the mammary secretions from the uninfected glands.
These results suggest that after an intramammary infusion of IL-8 has elicited inflammatory responses, it induces the prolonged secretion of elastase, inflammatory LDP, and IL-8, and that long-lasting IL-8-induced inflammatory reactions are involved in the pathogenesis of S.
aureus dry-period mastitis.
American Psychological Association (APA)
Watanabe, Atsushi& Hirota, Jiro& Shimizu, Shinya& Inumaru, Shigeki& Kimura, Kazuhiro. 2012. Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003165
Modern Language Association (MLA)
Watanabe, Atsushi…[et al.]. Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows. Veterinary Medicine International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1003165
American Medical Association (AMA)
Watanabe, Atsushi& Hirota, Jiro& Shimizu, Shinya& Inumaru, Shigeki& Kimura, Kazuhiro. Single Intramammary Infusion of Recombinant Bovine Interleukin-8 at Dry-Off Induces the Prolonged Secretion of Leukocyte Elastase, Inflammatory Lactoferrin-Derived Peptides, and Interleukin-8 in Dairy Cows. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003165