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Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis
Joint Authors
Azadmehr, Abbas
Bandehagh, Ali
Hajiaghaee, Reza
Shahneh, Fatemeh Zare
Baradaran, Behzad
Valiyari, Samira
Yaripour, Saeid
Source
Advances in Pharmacological and Pharmaceutical Sciences
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Echinophora platyloba DC plant (Khousharizeh) is one of the indigenous medicinal plants which is used as a food seasoning and medicine in Iran.
The objective of this study was to examine the in vitro cytotoxic activity and the mechanism of cell death of crude methanolic extracts prepared from Echinophora platyloba DC, on mouse fibrosarcoma cell line (WEHI-164).
Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay.
Cell death ELISA was employed to quantify the nucleosome production result from nuclear DNA fragmentation during apoptosis and determine whether the mechanism involves induction of apoptosis or necrosis.
The cell death was identified as apoptosis using terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) assay.
Our results demonstrated that the extract decreased cell viability, suppressed cell proliferation, and induced cell death in a time- and dose-dependent manner in WEHI-164 cells (IC50 = 196.673 ± 12.4 μg/mL) when compared with a chemotherapeutic anticancer drug, Toxol.
Observation proved that apoptosis was the major mechanism of cell death.
So the Echinophora platyloba DC extract was found to time- and dose-dependently inhibit the proliferation of fibrosarcoma cell possibly via an apoptosis-dependent pathway.
American Psychological Association (APA)
Shahneh, Fatemeh Zare& Valiyari, Samira& Azadmehr, Abbas& Hajiaghaee, Reza& Yaripour, Saeid& Bandehagh, Ali…[et al.]. 2013. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003214
Modern Language Association (MLA)
Shahneh, Fatemeh Zare…[et al.]. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis. Advances in Pharmacological and Pharmaceutical Sciences No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003214
American Medical Association (AMA)
Shahneh, Fatemeh Zare& Valiyari, Samira& Azadmehr, Abbas& Hajiaghaee, Reza& Yaripour, Saeid& Bandehagh, Ali…[et al.]. Inhibition of Growth and Induction of Apoptosis in Fibrosarcoma Cell Lines by Echinophora platyloba DC: In Vitro Analysis. Advances in Pharmacological and Pharmaceutical Sciences. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003214