Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin
Joint Authors
Zhao, Shuqiang
Zhang, Yu
Tian, Hong
Chen, Xiaofei
Cai, Di
Yao, Wenbing
Gao, Xiangdong
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Protein fusion technology is one of the most commonly used methods to extend the half-life of therapeutic proteins.
In this study, in order to prolong the half-life of Granulocyte colony stimulating factor (G-CSF), the domain III of human serum albumin (3DHSA) was genetically fused to the N-terminal of G-CSF.
The 3DHSA-G-CSF fusion gene was cloned into pPICZαA along with the open reading frame of the α-factor signal under the control of the AOX1 promoter.
The recombinant expression vector was transformed into Pichia pastoris GS115, and the recombinant strains were screened by SDS-PAGE.
As expected, the 3DHSA-G-CSF showed high binding affinity with HSA antibody and G-CSF antibody, and the natural N-terminal of 3DHSA was detected by N-terminal sequencing.
The bioactivity and pharmacokinetic studies of 3DHSA-G-CSF were respectively determined using neutropenia model mice and human G-CSF ELISA kit.
The results demonstrated that 3DHSA-G-CSF has the ability to increase the peripheral white blood cell (WBC) counts of neutropenia model mice, and the half-life of 3DHSA-G-CSF is longer than that of native G-CSF.
In conclusion, 3DHSA can be used to extend the half-life of G-CSF.
American Psychological Association (APA)
Zhao, Shuqiang& Zhang, Yu& Tian, Hong& Chen, Xiaofei& Cai, Di& Yao, Wenbing…[et al.]. 2013. Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003261
Modern Language Association (MLA)
Zhao, Shuqiang…[et al.]. Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003261
American Medical Association (AMA)
Zhao, Shuqiang& Zhang, Yu& Tian, Hong& Chen, Xiaofei& Cai, Di& Yao, Wenbing…[et al.]. Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003261
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003261