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A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Joint Authors
Xiong, Fei-Fei
Li, Ben-Shang
Zhang, Chun-Xiu
Xiong, Hui
Shen, Shu-Hong
Zhang, Qing-Hua
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Chromosome rearrangements and fusion genes present major portion of leukemogenesis and contribute to leukemic subtypes.
It is practical and helpful to detect the fusion genes in clinic diagnosis of leukemia.
Present application of reverse transcription polymerase chain reaction (RT-PCR) method to detect the fusion gene transcripts is effective, but time- and labor-consuming.
To set up a simple and rapid system, we established a method that combined multiplex RT-PCR and microarray.
We selected 15 clinically most frequently observed chromosomal rearrangements generating more than 50 fusion gene variants.
Chimeric reverse primers and chimeric PCR primers containing both gene-specific and universal sequences were applied in the procedure of multiplex RT-PCR, and then the PCR products hybridized with a designed microarray.
With this approach, among 200 clinic samples, 63 samples were detected to have gene rearrangements.
All the detected fusion genes positive and negative were validated with RT-PCR and Sanger sequencing.
Our data suggested that the RT-PCR-microarray pipeline could screen 15 partner gene pairs simultaneously at the same accuracy of the fusion gene detection with regular RT-PCR.
The pipeline showed effectiveness in multiple fusion genes screening in clinic samples.
American Psychological Association (APA)
Xiong, Fei-Fei& Li, Ben-Shang& Zhang, Chun-Xiu& Xiong, Hui& Shen, Shu-Hong& Zhang, Qing-Hua. 2013. A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1003320
Modern Language Association (MLA)
Xiong, Fei-Fei…[et al.]. A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1003320
American Medical Association (AMA)
Xiong, Fei-Fei& Li, Ben-Shang& Zhang, Chun-Xiu& Xiong, Hui& Shen, Shu-Hong& Zhang, Qing-Hua. A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1003320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003320