Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum
Joint Authors
Shen, Zhisen
Chen, Jingjing
Kang, Cheng
Gong, Changfeng
Zhu, Yabin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Porous polymeric scaffolds have been much investigated and applied in the field of tissue engineering research.
Poly(ester urethane) (PEU) scaffolds, comprising pores of 1–20 μm in diameter on one surface and ≥200 μm on the opposite surface and in bulk, were fabricated using phase separation method for hypopharyngeal tissue engineering.
The scaffolds were grafted with silk fibroin (SF) generated from natural silkworm cocoon to enhance the scaffold’s hydrophilicity and further improve cytocompatibility to both primary epithelial cells (ECs) and fibroblasts of human hypopharynx tissue.
Coculture of ECs and fibroblasts was conducted on the SF-grafted PEU scaffold (PEU-SF) to evaluate its in vitro cytocompatibility.
After co-culture for 14 days, ECs were lined on the scaffold surface while fibroblasts were distributed in scaffold bulk.
The results of in vivo investigation showed that PEU porous scaffold possessed good biocompatibility after it was grafted by silk fibroin.
SF grafting improved the cell/tissue infiltration into scaffold bulk.
Thus, PEU-SF porous scaffold is expected to be a good candidate to support the hypopharynx regeneration.
American Psychological Association (APA)
Shen, Zhisen& Chen, Jingjing& Kang, Cheng& Gong, Changfeng& Zhu, Yabin. 2013. Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003351
Modern Language Association (MLA)
Shen, Zhisen…[et al.]. Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1003351
American Medical Association (AMA)
Shen, Zhisen& Chen, Jingjing& Kang, Cheng& Gong, Changfeng& Zhu, Yabin. Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003351
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003351