Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig
Joint Authors
Kwon, Dae-Jin
Jeon, Hyelena
Oh, Keon Bong
Ock, Sun-A
Im, Gi-Sun
Lee, Sung-Soo
Im, Seok Ki
Lee, Jeong-Woong
Oh, Sung-Jong
Park, Jin-Ki
Hwang, Seongsoo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The generation and application of porcine induced pluripotent stem cells (iPSCs) may enable the testing for safety and efficacy of therapy in the field of human regenerative medicine.
Here, the generation of iPSCs from the Massachusetts General Hospital miniature pig (MGH minipig) established for organ transplantation studies is reported.
Fibroblasts were isolated from the skin of the ear of a 10-day-old MGH minipig and transduced with a cocktail of six human factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, and LIN28.
Two distinct types of iPSCs were generated that were positive for alkaline phosphatase activity, as well as the classical pluripotency markers: Oct4, Nanog, Sox2, and the surface marker Ssea-1.
Only one of two porcine iPSC lines differentiated into three germ layers both in vitro and in vivo.
Western blot analysis showed that the porcine iPSCs were dependent on LIF or BMP-4 to sustain self-renewal and pluripotency.
In conclusion, the results showed that human pluripotent factors could reprogram porcine ear fibroblasts into the pluripotent state.
These cells may provide a useful source of cells that could be used for the treatment of degenerative and genetic diseases and agricultural research and application.
American Psychological Association (APA)
Kwon, Dae-Jin& Jeon, Hyelena& Oh, Keon Bong& Ock, Sun-A& Im, Gi-Sun& Lee, Sung-Soo…[et al.]. 2013. Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003378
Modern Language Association (MLA)
Kwon, Dae-Jin…[et al.]. Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1003378
American Medical Association (AMA)
Kwon, Dae-Jin& Jeon, Hyelena& Oh, Keon Bong& Ock, Sun-A& Im, Gi-Sun& Lee, Sung-Soo…[et al.]. Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003378