Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets
Joint Authors
Song, Yimin
Wei, Zheng
Song, Chun
Xie, Shanshan
Feng, Jinfa
Fan, Jiehou
Zhang, Zengling
Shi, Yubo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The in vitro culture of pancreatic islets reduces their immunogenicity and prolongs their availability for transplantation.
Both simulated microgravity (sMG) and a polyglycolic acid scaffold (PGA) are believed to confer advantages to cell culture.
Here, we evaluated the effects of sMG combined with a PGA on the viability, insulin-producing activity and morphological alterations of pancreatic islets.
Under PGA-sMG conditions, the purity of the islets was ≥85%, and the islets had a higher survival rate and an increased ability to secrete insulin compared with islets cultured alone in the static, sMG, or PGA conditions.
In addition, morphological analysis under scanning electron microscopy (SEM) revealed that the PGA-sMG treatment preserved the integral structure of the islets and facilitated islet adhesion to the scaffolds.
These results suggest that PGA-sMG coculture has the potential to improve the viability and function of islets in vitro and provides a promising method for islet transplantation.
American Psychological Association (APA)
Song, Yimin& Wei, Zheng& Song, Chun& Xie, Shanshan& Feng, Jinfa& Fan, Jiehou…[et al.]. 2013. Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003446
Modern Language Association (MLA)
Song, Yimin…[et al.]. Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003446
American Medical Association (AMA)
Song, Yimin& Wei, Zheng& Song, Chun& Xie, Shanshan& Feng, Jinfa& Fan, Jiehou…[et al.]. Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003446