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Use of Insulin to Increase Epiblast Cell Number: Towards a New Approach for Improving ESC Isolation from Human Embryos
Joint Authors
Campbell, Jared M.
Lane, Michelle
Vassiliev, Ivan
Nottle, Mark B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Human embryos donated for embryonic stem cell (ESC) derivation have often been cryopreserved for 5–10 years.
As a consequence, many of these embryos have been cultured in media now known to affect embryo viability and the number of ESC progenitor epiblast cells.
Historically, these conditions supported only low levels of blastocyst development necessitating their transfer or cryopreservation at the 4–8-cell stage.
As such, these embryos are donated at the cleavage stage and require further culture to the blastocyst stage before hESC derivation can be attempted.
These are generally of poor quality, and, consequently, the efficiency of hESC derivation is low.
Recent work using a mouse model has shown that the culture of embryos from the cleavage stage with insulin to day 6 increases the blastocyst epiblast cell number, which in turn increases the number of pluripotent cells in outgrowths following plating, and results in an increased capacity to give rise to ESCs.
These findings suggest that culture with insulin may provide a strategy to improve the efficiency with which hESCs are derived from embryos donated at the cleavage stage.
American Psychological Association (APA)
Campbell, Jared M.& Lane, Michelle& Vassiliev, Ivan& Nottle, Mark B.. 2013. Use of Insulin to Increase Epiblast Cell Number: Towards a New Approach for Improving ESC Isolation from Human Embryos. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003447
Modern Language Association (MLA)
Campbell, Jared M.…[et al.]. Use of Insulin to Increase Epiblast Cell Number: Towards a New Approach for Improving ESC Isolation from Human Embryos. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003447
American Medical Association (AMA)
Campbell, Jared M.& Lane, Michelle& Vassiliev, Ivan& Nottle, Mark B.. Use of Insulin to Increase Epiblast Cell Number: Towards a New Approach for Improving ESC Isolation from Human Embryos. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003447