Trace Level Determination of Polyether Ionophores in Feed
Joint Authors
Rokka, Mervi
Jestoi, Marika
Peltonen, Kimmo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A liquid chromatography-mass spectrometric method was developed and validated to determine six polyether ionophores (lasalocid sodium, monensin sodium, salinomycin sodium, narasin, maduramicin ammonium alpha, and semduramicin sodium) in feed samples.
The method developed was very straightforward, involving extraction with 84% acetonitrile of the coccidiostats from the feed samples and filtration of the raw extract prior to chromatographic analysis.
Method validation included the determination of selectivity, linearity, specificity, repeatability, the limit of detection, limit of quantification, decision limit (CCα), detection capability (CCβ), and recovery.
Feed samples from the Finnish national feed control programme and suspected carry-over samples from a feed manufacturer were analysed in parallel with an existing liquid chromatography method coupled with ultraviolet detection.
All feed control samples were negative in LC-UV, but with the developed MS method, monensin, salinomycin, and narasin were detected at concentrations of <0.025–0.73 mg/kg, <0.025–0.027 mg/kg, and <0.025–1.6 mg/kg, respectively.
In suspected carry-over samples after an output of 2.0 tonnes of unmedicated feed in the pelletizer line, the concentrations of monensin, salinomycin, and narasin varied from undetected to 16 mg/kg.
In the mixer line, after 3.2 tonnes of unmedicated feed output, the concentrations of monensin, salinomycin, and narasin varied from undetected to 2.4 mg/kg.
American Psychological Association (APA)
Rokka, Mervi& Jestoi, Marika& Peltonen, Kimmo. 2012. Trace Level Determination of Polyether Ionophores in Feed. BioMed Research International،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003448
Modern Language Association (MLA)
Rokka, Mervi…[et al.]. Trace Level Determination of Polyether Ionophores in Feed. BioMed Research International No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-1003448
American Medical Association (AMA)
Rokka, Mervi& Jestoi, Marika& Peltonen, Kimmo. Trace Level Determination of Polyether Ionophores in Feed. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-1003448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003448