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Correlation between Phylogroups and Intracellular Proteomes of Propionibacterium acnes and Differences in the Protein Expression Profiles between Anaerobically and Aerobically Grown Cells
Joint Authors
Dekio, Itaru
Culak, Renata
Fang, Min
Ball, Graham
Gharbia, Saheer
Shah, Haroun N.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Propionibacterium acnes is one of the dominant commensals on the human skin and also an opportunistic pathogen in relation to acne, sarcoidosis, prostate cancer, and various infections.
Recent investigations using housekeeping and virulence genes have revealed that the species consists of three major evolutionary clades (types I, II, and III).
In order to investigate protein expression differences between these phylogroups, proteomic profiles of 21 strains of P.
acnes were investigated.
The proteins extracted from cells cultured under anaerobic and aerobic conditions were analysed using a SELDI-TOF mass spectrometer, high-resolution capillary gel electrophoresis, and LC-MS/ MS.
The SELDI spectral profiles were visualised as a heat map and a dendrogram, which resulted in four proteomic groups.
Strains belonging to type I were represented in the proteome Group A, while Group B contained type III strains.
Groups C and D contained mixtures of types I and II.
Each of these groups was not influenced by differences in culture conditions.
Under anoxic growth conditions, a type IB strain yielded high expressions of some proteins, such as methylmalonyl-CoA epimerase and the Christie-Atkins-Munch-Petersen (CAMP) factor.
The present study revealed good congruence between genomic and proteomic data suggesting that the microenvironment of each subtype may influence protein expression.
American Psychological Association (APA)
Dekio, Itaru& Culak, Renata& Fang, Min& Ball, Graham& Gharbia, Saheer& Shah, Haroun N.. 2013. Correlation between Phylogroups and Intracellular Proteomes of Propionibacterium acnes and Differences in the Protein Expression Profiles between Anaerobically and Aerobically Grown Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003452
Modern Language Association (MLA)
Dekio, Itaru…[et al.]. Correlation between Phylogroups and Intracellular Proteomes of Propionibacterium acnes and Differences in the Protein Expression Profiles between Anaerobically and Aerobically Grown Cells. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003452
American Medical Association (AMA)
Dekio, Itaru& Culak, Renata& Fang, Min& Ball, Graham& Gharbia, Saheer& Shah, Haroun N.. Correlation between Phylogroups and Intracellular Proteomes of Propionibacterium acnes and Differences in the Protein Expression Profiles between Anaerobically and Aerobically Grown Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003452