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Effect of Ex Vivo Culture Conditions on Immunosuppression by Human Mesenchymal Stem Cells
Joint Authors
Lee, Myoung Woo
Kim, Dae Seong
Ryu, Somi
Jang, In Keun
Kim, Hye Jin
Yang, Jin Mo
Lee, Doo-Hoon
Lee, Soo Hyun
Son, Meong Hi
Cheuh, Hee Won
Jung, Hye Lim
Yoo, Keon Hee
Sung, Ki Woong
Koo, Hong Hoe
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs.
Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density.
The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P<0.05, FC>2).
The genes that were highly expressed in MSCs largely corresponded to chemotaxis, inflammation, and immune responses, indicating direct or indirect involvement in immunomodulatory functions.
Specifically, PTGES and ULBP1 were up-regulated in MSCs harvested at high density.
Treatment of MSCs with PTGES or ULBP1 siRNA reversed their inhibition of T-cell proliferation in vitro.
The culture conditions such as cell confluence at harvest seem to be important for gene expression profile of MSCs; therefore, the results of this study may provide useful guidelines for the harvest of MSCs that can appropriately suppress the immune response.
American Psychological Association (APA)
Lee, Myoung Woo& Kim, Dae Seong& Ryu, Somi& Jang, In Keun& Kim, Hye Jin& Yang, Jin Mo…[et al.]. 2013. Effect of Ex Vivo Culture Conditions on Immunosuppression by Human Mesenchymal Stem Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003513
Modern Language Association (MLA)
Lee, Myoung Woo…[et al.]. Effect of Ex Vivo Culture Conditions on Immunosuppression by Human Mesenchymal Stem Cells. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003513
American Medical Association (AMA)
Lee, Myoung Woo& Kim, Dae Seong& Ryu, Somi& Jang, In Keun& Kim, Hye Jin& Yang, Jin Mo…[et al.]. Effect of Ex Vivo Culture Conditions on Immunosuppression by Human Mesenchymal Stem Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003513
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003513