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Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions
Joint Authors
Fujisaki, Masami
Nagoshi, Tomohisa
Nishikawa, Tetsuo
Date, Taro
Yoshimura, Michihiro
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In addition to classical adrenal cortical biosynthetic pathway, there is increasing evidence that aldosterone is produced in extra-adrenal tissues.
Although we previously reported aldosterone production in the heart, the concept of cardiac aldosterone synthesis remains controversial.
This is partly due to lack of established experimental models representing aldosterone synthase (CYP11B2) expression in robustly reproducible fashion.
We herein investigated suitable conditions in neonatal rat cardiomyocytes (NRCMs) culture system producing CYP11B2 with considerable efficacy.
NRCMs were cultured with various glucose doses for 2–24 hours.
CYP11B2 mRNA expression and aldosterone concentrations secreted from NRCMs were determined using real-time PCR and enzyme immunoassay, respectively.
We found that suitable conditions for CYP11B2 induction included four-hour incubation with high glucose conditions.
Under these particular conditions, CYP11B2 expression, in accordance with aldosterone secretion, was significantly increased compared to those observed in the cells cultured under standard-glucose condition.
Angiotensin II receptor blocker partially inhibited this CYP11B2 induction, suggesting that there is local renin-angiotensin-aldosterone system activation under high glucose conditions.
The suitable conditions for CYP11B2 induction in NRCMs culture system are now clarified: high-glucose conditions with relatively brief period of culture promote CYP11B2 expression in cardiomyocytes.
The current system will help to accelerate further progress in research on cardiac tissue aldosterone synthesis.
American Psychological Association (APA)
Fujisaki, Masami& Nagoshi, Tomohisa& Nishikawa, Tetsuo& Date, Taro& Yoshimura, Michihiro. 2013. Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003571
Modern Language Association (MLA)
Fujisaki, Masami…[et al.]. Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1003571
American Medical Association (AMA)
Fujisaki, Masami& Nagoshi, Tomohisa& Nishikawa, Tetsuo& Date, Taro& Yoshimura, Michihiro. Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003571