![](/images/graphics-bg.png)
Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression
Joint Authors
Peng, Haixia
Long, Feng
Wu, Zhiyuan
Chu, Yimin
Li, Ji
Kuai, Rong
Zhang, Jing
Kang, Zhihua
Zhang, Xinju
Guan, Ming
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
DLC-1 is a tumor suppressor gene frequently silenced in human cancers.
However, the pathogenicity of DLC-1 epigenetic silencing in the mucosa-adenoma-carcinoma transformation process of colorectal cancer (CRC) has not been studied.
Methods.
Promoter methylation status of DLC-1 was evaluated in 4 human CRC cell lines, 48 normal mucosa, 57 adenomas, and 80 CRC tissues with methylation-sensitive high-resolution melting analysis (MS-HRMA), while the mRNA expression was examined by qPCR.
HRMA was utilized to detect the KRAS codon 12, 13 and BRAF V600Emutations.
Results.
Partial (1%–10%) and extensive (10%–100%) DLC-1 promoter methylations were observed in 10% and 0% of normal mucosa, 46% and 14% of adenomas, and 60% and 36% of CRCs, respectively.
The promoter methylation of DLC-1 was related with the reduction of gene expression and the advanced Duke’s stages (Stage C and D).
DLC-1 promoter methylation and KRAS mutations are common concurrent pathological alternations.
Conclusions.
Epigenetic alternation plays a key role in the transcriptional silencing of DLC-1.
It is also an independent risk factor related to the carcinogenesis of colorectal tumors and spans over its pathogenesis process.
Therefore, DLC-1 promoter methylation quantitation may have a promising significance in the evaluation and management of CRC patients.
American Psychological Association (APA)
Peng, Haixia& Long, Feng& Wu, Zhiyuan& Chu, Yimin& Li, Ji& Kuai, Rong…[et al.]. 2012. Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003629
Modern Language Association (MLA)
Peng, Haixia…[et al.]. Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003629
American Medical Association (AMA)
Peng, Haixia& Long, Feng& Wu, Zhiyuan& Chu, Yimin& Li, Ji& Kuai, Rong…[et al.]. Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003629