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Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells
Joint Authors
D’Elia, Piera
Ionta, Vittoria
Chimenti, Isotta
Angelini, Francesco
Miraldi, Fabio
Pala, Alessandro
Messina, Elisa
Giacomello, Alessandro
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
IGF-binding proteins (IGFBPs) and their proteases regulate IGFs bioavailability in multiple tissues.
Pregnancy-associated plasma protein A (PAPP-A) is a protease acting by cleaving IGFBP2, 4, and 5, regulating local bioavailability of IGFs.
We have previously shown that IGFs and IGFBPs are produced by human adult cardiac progenitor cells (haCPCs) and that IGF-1 exerts paracrine therapeutic effects in cardiac cell therapy with CPCs.
Using immunofluorescence and enzyme immunoassays, we firstly report that PAPP-A is produced and secreted in surprisingly high amounts by haCPCs.
In particular, the homodimeric, enzymatically active, PAPP-A is secreted in relevant concentrations in haCPC-conditioned media, while the enzymatically inactive PAPPA/proMBP complex is not detectable in the same media.
Furthermore, we show that both homodimeric PAPP-A and proMBP can be detected as cell associated, suggesting that the previously described complex formation at the cell surface does not occur easily, thus positively affecting IGF signalling.
Therefore, our results strongly support the importance of PAPP-A for the IGFs/IGFBPs/PAPP-A axis in CPCs biology.
American Psychological Association (APA)
D’Elia, Piera& Ionta, Vittoria& Chimenti, Isotta& Angelini, Francesco& Miraldi, Fabio& Pala, Alessandro…[et al.]. 2013. Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003657
Modern Language Association (MLA)
D’Elia, Piera…[et al.]. Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003657
American Medical Association (AMA)
D’Elia, Piera& Ionta, Vittoria& Chimenti, Isotta& Angelini, Francesco& Miraldi, Fabio& Pala, Alessandro…[et al.]. Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003657