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Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae)
Joint Authors
Bastos, Jairo Kennup
Perazzo, Fabio Ferreira
Lopes, Andrey P.
Bagatela, Bianca S.
Rosa, Paulo C. P.
Nanayakkara, Dhammika N. P.
Carlos Tavares Carvalho, José
Maistro, Edson L.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The crude ethanolic extract from aerial parts of Pothomorphe umbellata L.
(Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells.
The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50=1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50=4.5 μg/mL), methylene chloride (IC50=5.9 μg/mL), ethyl acetate (IC50=8.0 μg/mL), 4-nerolidylcatechol (IC50=8.6 μg/mL), and the sterol fractions (IC50>12.5 μg/mL).
Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL.
4-Nerolidylcatechol (IC50=0.4 μg/mL) and methylene chloride fraction (IC50=2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol.
Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective.
American Psychological Association (APA)
Lopes, Andrey P.& Bagatela, Bianca S.& Rosa, Paulo C. P.& Nanayakkara, Dhammika N. P.& Carlos Tavares Carvalho, José& Maistro, Edson L.…[et al.]. 2012. Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae). BioMed Research International،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1003705
Modern Language Association (MLA)
Lopes, Andrey P.…[et al.]. Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae). BioMed Research International No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1003705
American Medical Association (AMA)
Lopes, Andrey P.& Bagatela, Bianca S.& Rosa, Paulo C. P.& Nanayakkara, Dhammika N. P.& Carlos Tavares Carvalho, José& Maistro, Edson L.…[et al.]. Antioxidant and Cytotoxic Effects of Crude Extract, Fractions and 4-Nerolidylcathecol from Aerial Parts of Pothomorphe umbellata L. (Piperaceae). BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1003705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003705