Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells
Joint Authors
Prosen, Lara
Prijic, Sara
Music, Branka
Lavrencak, Jaka
Cemazar, Maja
Sersa, Gregor
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Magnetofection is a nanoparticle-mediated approach for transfection of cells, tissues, and tumors.
Specific interest is in using superparamagnetic iron oxide nanoparticles (SPIONs) as delivery system of therapeutic genes.
Magnetofection has already been described in some proof-of-principle studies; however, fine tuning of the synthesis of SPIONs is necessary for its broader application.
Physicochemical properties of SPIONs, synthesized by the co-precipitation in an alkaline aqueous medium, were tested after varying different parameters of the synthesis procedure.
The storage time of iron(II) sulfate salt, the type of purified water, and the synthesis temperature did not affect physicochemical properties of SPIONs.
Also, varying the parameters of the synthesis procedure did not influence magnetofection efficacy.
However, for the pronounced gene expression encoded by plasmid DNA it was crucial to functionalize poly(acrylic) acid-stabilized SPIONs (SPIONs-PAA) with polyethyleneimine (PEI) without the adjustment of its elementary alkaline pH water solution to the physiological pH.
In conclusion, the co-precipitation of iron(II) and iron(III) sulfate salts with subsequent PAA stabilization, PEI functionalization, and plasmid DNA binding is a robust method resulting in a reproducible and efficient magnetofection.
To achieve high gene expression is important, however, the pH of PEI water solution for SPIONs-PAA functionalization, which should be in the alkaline range.
American Psychological Association (APA)
Prosen, Lara& Prijic, Sara& Music, Branka& Lavrencak, Jaka& Cemazar, Maja& Sersa, Gregor. 2013. Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003713
Modern Language Association (MLA)
Prosen, Lara…[et al.]. Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1003713
American Medical Association (AMA)
Prosen, Lara& Prijic, Sara& Music, Branka& Lavrencak, Jaka& Cemazar, Maja& Sersa, Gregor. Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1003713
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003713