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Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1 in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells
Joint Authors
Kim, Eun-Cheol
Jung, Ha-Jin
Lee, Seung-Ho
Lee, Deok-Won
Lee, Young-Man
Auh, Q-Schick
Kim, Jun-Yeol
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-02
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Although previous studies have shown that mollugin, a bioactive phytochemical isolated from Rubia cordifolia L.
(Rubiaceae), exhibits antitumor effects, its biological activity in oral cancer has not been reported.
We thus investigated the effects and putative mechanism of apoptosis induced by mollugin in human oral squamous cell carcinoma cells (OSCCs).
Results show that mollugin induces cell death in a dose-dependent manner in primary and metastatic OSCCs.
Mollugin-induced cell death involved apoptosis, characterized by the appearance of nuclear shrinkage, flow cytometric analysis of sub-G1 phase arrest, and annexin V-FITC and propidium iodide staining.
Western blot analysis and RT-PCR revealed that mollugin suppressed activation of NF-κB and NF-κB-dependent gene products involved in antiapoptosis (Bcl-2 and Bcl-xl), invasion (MMP-9 and ICAM-1), and angiogenesis (FGF-2 and VEGF).
Furthermore, mollugin induced the activation of p38, ERK, and JNK and the expression of heme oxygenase-1 (HO-1) and nuclear factor E2–related factor 2 (Nrf2).
Mollugin-induced growth inhibition and apoptosis of HO-1 were reversed by an HO-1 inhibitor and Nrf2 siRNA.
Collectively, this is the first report to demonstrate the effectiveness of mollugin as a candidate for a chemotherapeutic agent in OSCCs via the upregulation of the HO-1 and Nrf2 pathways and the downregulation of NF-κB.
American Psychological Association (APA)
Lee, Young-Man& Auh, Q-Schick& Lee, Deok-Won& Kim, Jun-Yeol& Jung, Ha-Jin& Lee, Seung-Ho…[et al.]. 2013. Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1 in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1003717
Modern Language Association (MLA)
Lee, Young-Man…[et al.]. Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1 in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells. BioMed Research International No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-1003717
American Medical Association (AMA)
Lee, Young-Man& Auh, Q-Schick& Lee, Deok-Won& Kim, Jun-Yeol& Jung, Ha-Jin& Lee, Seung-Ho…[et al.]. Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1 in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-1003717
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003717