miR-1-Mediated Induction of Cardiogenesis in Mesenchymal Stem Cells via Downregulation of Hes-1
Joint Authors
Huang, Feng
Tang, Liang
Fang, Zhen-fei
Hu, Xin-qun
Pan, Jia-yi
Zhou, Sheng-hua
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
MicroRNAs (miRNAs, miRs) have the potential to control stem cells fate decisions.
The cardiac- and skeletal-muscle-specific miRNA, miR-1, can regulate embryonic stem cells differentiation to cardiac lineage by suppressing gene expression of alternative lineages.
Accordingly, we hypothesized that overexpression of miR-1 may also promote cardiac gene expression in mesenchymal stem cells.
Since Notch signaling could inhibit muscle differentiation, a process in contrast with the effect of miR-1, miR-1-mediated repression of Notch signaling may contribute to the observed effects of miR-1 in mesenchymal stem cells.
Thus, mesenchymal stem cells were infected by lentiviral vectors carrying miR-1, and cells expressing miR-1 were selected.
Alterations in Notch signaling and cardiomyocyte markers, Nkx2.5, GATA-4, cTnT, and CX43, were identified by Western blot in the infected cells on days 1, 7, and 14.
Our study showed that the downstream target molecule of Notch pathway, Hes-1, was obviously decreased in mesenchymal stem cells modified with miR-1, and overexpression of miR-1 promotes the specific cardiac gene expression in the infected cells.
Knockdown of Hes-1 leads to the same effects on cell lineage decisions.
Our results indicated that miR-1 promotes the differentiation of MSCs into cardiac lineage in part due to negative regulation of Hes-1.
American Psychological Association (APA)
Huang, Feng& Tang, Liang& Fang, Zhen-fei& Hu, Xin-qun& Pan, Jia-yi& Zhou, Sheng-hua. 2012. miR-1-Mediated Induction of Cardiogenesis in Mesenchymal Stem Cells via Downregulation of Hes-1. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003731
Modern Language Association (MLA)
Huang, Feng…[et al.]. miR-1-Mediated Induction of Cardiogenesis in Mesenchymal Stem Cells via Downregulation of Hes-1. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003731
American Medical Association (AMA)
Huang, Feng& Tang, Liang& Fang, Zhen-fei& Hu, Xin-qun& Pan, Jia-yi& Zhou, Sheng-hua. miR-1-Mediated Induction of Cardiogenesis in Mesenchymal Stem Cells via Downregulation of Hes-1. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003731