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Study on Characteristics of Chemokine CXCL10 Gene Cloned from cDNA Expression Library of Ujumqin Sheep
Joint Authors
Hu, P. F.
Li, X. C.
Lei, N.
Lan, X. Y.
Zhao, Q. J.
Guan, W. J.
Ma, Y. H.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chemokines were a major regulator of body’s inflammatory and immune responses.
In this study, the cDNA fragment of chemokine CXC ligand 10 (CXCL10) was cloned from the Ujumqin sheep ear marginal tissue cDNA expression library; the CXCL10 gene had 103 amino acids and a molecular weight of 11.47 kDa, and it shared a high homology among cattle, sheep, and goat, while a low homology compared with mouse.
The CXCL10 protein had 4 conservative cysteine residues, located in 28, 30, 55, and 72 sites.
The expression pattern and intracellular distribution of recombinant CXCL10 proteins in Ujumqin sheep fibroblast cells showed that there were green fluorescence signals both in cytoplasm and nucleolus after 24 h of transfection, the number of positive cells was increased with time, the peak level of fluorescence signal was reached after 48 h of transfection and the transfection efficiency was 33.3%; there was a significant decrease in fluorescence intensity after 72 h of transfection.
Expression of recombinant CXCL10 gene in Escherichia coli had a time- and temperature-dependency on the amount of protein expression, and a small quantity of inducer was needed.
American Psychological Association (APA)
Hu, P. F.& Li, X. C.& Lei, N.& Lan, X. Y.& Zhao, Q. J.& Guan, W. J.…[et al.]. 2013. Study on Characteristics of Chemokine CXCL10 Gene Cloned from cDNA Expression Library of Ujumqin Sheep. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003733
Modern Language Association (MLA)
Hu, P. F.…[et al.]. Study on Characteristics of Chemokine CXCL10 Gene Cloned from cDNA Expression Library of Ujumqin Sheep. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003733
American Medical Association (AMA)
Hu, P. F.& Li, X. C.& Lei, N.& Lan, X. Y.& Zhao, Q. J.& Guan, W. J.…[et al.]. Study on Characteristics of Chemokine CXCL10 Gene Cloned from cDNA Expression Library of Ujumqin Sheep. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003733