Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro
Joint Authors
Li, Tian Xia
Yuan, Jie
Chen, Yan
Pan, Li Jie
Song, Chun
Bi, Liang Jia
Jiao, Xiao Hui
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The easily accessible mesenchymal stem cells in the Wharton's jelly of human umbilical cord tissue (hUCMSCs) have excellent proliferation and differentiation potential, but it remains unclear whether hUCMSCs can differentiate into odontoblasts.
In this study, mesenchymal stem cells were isolated from the Wharton's jelly of human umbilical cord tissue using the simple method of tissue blocks culture attachment.
UCMSC surface marker expression was then evaluated for the isolated cells using flow cytometry.
The third-passage hUCMSCs induced by conditioned medium from developing tooth germ cells (TGC-CM) displayed high alkaline phosphatase (ALP) levels (P<0.001), an enhanced ability to proliferate (P<0.05), and the presence of mineralized nodules.
These effects were not observed in cells treated with regular medium.
After induction of hUCMSCs, the results of reverse transcriptional polymerase chain reaction (PCR) indicated that the dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP1) genes were significantly tested.
Additionally, dentin sialoprotein (DSP) and DMP1 demonstrated significant levels of staining in an immunofluorescence analysis.
In contrast, the control cells failed to display the characteristics of odontoblasts.
Taken together, these results suggest that hUCMSCs can be induced to differentiate into odontoblast-like cells with TGC-CM and provide a novel strategy for tooth regeneration research.
American Psychological Association (APA)
Li, Tian Xia& Yuan, Jie& Chen, Yan& Pan, Li Jie& Song, Chun& Bi, Liang Jia…[et al.]. 2013. Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003734
Modern Language Association (MLA)
Li, Tian Xia…[et al.]. Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003734
American Medical Association (AMA)
Li, Tian Xia& Yuan, Jie& Chen, Yan& Pan, Li Jie& Song, Chun& Bi, Liang Jia…[et al.]. Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003734