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HTR8SVneo Cells Display Trophoblast Progenitor Cell-Like Characteristics Indicative of Self-Renewal, Repopulation Activity, and Expression of “Stemness-” Associated Transcription Factors
Joint Authors
Weber, Maja
Knoefler, Ilka
Schleussner, Ekkehard
Markert, Udo R.
Fitzgerald, Justine S.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
JEG3 is a choriocarcinoma—and HTR8/SVneo a transformed extravillous trophoblast—cell line often used to model the physiologically invasive extravillous trophoblast.
Past studies suggest that these cell lines possess some stem or progenitor cell characteristics.
Aim was to study whether these cells fulfill minimum criteria used to identify stem-like (progenitor) cells.
In summary, we found that the expression profile of HTR8/SVneo (CDX2+, NOTCH1+, SOX2+, NANOG+, and OCT-) is distinct from JEG3 (CDX2+ and NOTCH1+) as seen only in human-serum blocked immunocytochemistry.
This correlates with HTR8/SVneo’s self-renewal capacities, as made visible via spheroid formation and multi-passagability in hanging drops protocols paralleling those used to maintain embryoid bodies.
JEG3 displayed only low propensity to form and reform spheroids.
HTR8/SVneo spheroids migrated to cover and seemingly repopulate human chorionic villi during confrontation cultures with placental explants in hanging drops.
We conclude that HTR8/SVneo spheroid cells possess progenitor cell traits that are probably attained through corruption of “stemness-” associated transcription factor networks.
Furthermore, trophoblastic cells are highly prone to unspecific binding, which is resistant to conventional blocking methods, but which can be alleviated through blockage with human serum.
American Psychological Association (APA)
Weber, Maja& Knoefler, Ilka& Schleussner, Ekkehard& Markert, Udo R.& Fitzgerald, Justine S.. 2013. HTR8SVneo Cells Display Trophoblast Progenitor Cell-Like Characteristics Indicative of Self-Renewal, Repopulation Activity, and Expression of “Stemness-” Associated Transcription Factors. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003776
Modern Language Association (MLA)
Weber, Maja…[et al.]. HTR8SVneo Cells Display Trophoblast Progenitor Cell-Like Characteristics Indicative of Self-Renewal, Repopulation Activity, and Expression of “Stemness-” Associated Transcription Factors. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003776
American Medical Association (AMA)
Weber, Maja& Knoefler, Ilka& Schleussner, Ekkehard& Markert, Udo R.& Fitzgerald, Justine S.. HTR8SVneo Cells Display Trophoblast Progenitor Cell-Like Characteristics Indicative of Self-Renewal, Repopulation Activity, and Expression of “Stemness-” Associated Transcription Factors. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003776
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003776