Degradation of Diuron by Phanerochaete chrysosporium: Role of Ligninolytic Enzymes and Cytochrome P450
Joint Authors
Coelho-Moreira, Jaqueline da Silva
Bracht, Adelar
Souza, Aline Cristine da Silva de
Oliveira, Roselene Ferreira
Sá-Nakanishi, Anacharis Babeto de
Souza, Cristina Giatti Marques de
Peralta, Rosane Marina
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The white-rot fungus Phanerochaete chrysosporium was investigated for its capacity to degrade the herbicide diuron in liquid stationary cultures.
The presence of diuron increased the production of lignin peroxidase in relation to control cultures but only barely affected the production of manganese peroxidase.
The herbicide at the concentration of 7 μg/mL did not cause any reduction in the biomass production and it was almost completely removed after 10 days.
Concomitantly with the removal of diuron, two metabolites, DCPMU [1-(3,4-dichlorophenyl)-3-methylurea] and DCPU [(3,4-dichlorophenyl)urea], were detected in the culture medium at the concentrations of 0.74 μg/mL and 0.06 μg/mL, respectively.
Crude extracellular ligninolytic enzymes were not efficient in the in vitro degradation of diuron.
In addition, 1-aminobenzotriazole (ABT), a cytochrome P450 inhibitor, significantly inhibited both diuron degradation and metabolites production.
Significant reduction in the toxicity evaluated by the Lactuca sativa L.
bioassay was observed in the cultures after 10 days of cultivation.
In conclusion, P.
chrysosporium can efficiently metabolize diuron without the accumulation of toxic products.
American Psychological Association (APA)
Coelho-Moreira, Jaqueline da Silva& Bracht, Adelar& Souza, Aline Cristine da Silva de& Oliveira, Roselene Ferreira& Sá-Nakanishi, Anacharis Babeto de& Souza, Cristina Giatti Marques de…[et al.]. 2013. Degradation of Diuron by Phanerochaete chrysosporium: Role of Ligninolytic Enzymes and Cytochrome P450. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003797
Modern Language Association (MLA)
Coelho-Moreira, Jaqueline da Silva…[et al.]. Degradation of Diuron by Phanerochaete chrysosporium: Role of Ligninolytic Enzymes and Cytochrome P450. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003797
American Medical Association (AMA)
Coelho-Moreira, Jaqueline da Silva& Bracht, Adelar& Souza, Aline Cristine da Silva de& Oliveira, Roselene Ferreira& Sá-Nakanishi, Anacharis Babeto de& Souza, Cristina Giatti Marques de…[et al.]. Degradation of Diuron by Phanerochaete chrysosporium: Role of Ligninolytic Enzymes and Cytochrome P450. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003797
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003797