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A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences
Joint Authors
Krimitzas, Antonios
Pyrri, Ioanna
Kouvelis, Vassili N.
Kapsanaki-Gotsi, Evangelia
Typas, Milton A.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-09
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus.
The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes (β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)) and two mitochondrial genes (small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)) and, where available, related sequences from databases.
The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti, and Zonati, with few uncertainties.
The combined use of the three commonly employed nuclear genes (benA, rpb2, and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit resolved several taxonomic ambiguities.
A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony, and Bayesian methods.
The strains examined formed seven well-supported clades within the genus Aspergillus.
Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding of phylogenetic relationships within this genus.
American Psychological Association (APA)
Krimitzas, Antonios& Pyrri, Ioanna& Kouvelis, Vassili N.& Kapsanaki-Gotsi, Evangelia& Typas, Milton A.. 2013. A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1003817
Modern Language Association (MLA)
Krimitzas, Antonios…[et al.]. A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1003817
American Medical Association (AMA)
Krimitzas, Antonios& Pyrri, Ioanna& Kouvelis, Vassili N.& Kapsanaki-Gotsi, Evangelia& Typas, Milton A.. A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1003817
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003817