Cratylia mollis 1, 4 Lectin: A New Biotechnological Tool in IL-6, IL-17A, IL-22, and IL-23 Induction and Generation of Immunological Memory
Joint Authors
de Melo Rego, Moacyr Jesus Barreto
Oliveira, Priscilla Stela Santana de
Rêgo, Moacyr Jesus Barreto de Melo
da Silva, Rafael Ramos
Cavalcanti, Mariana Brayner
Galdino, Suely Lins
Correia, Maria Tereza dos Santos
Pitta, Maira Galdino da Rocha
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-17
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Cratylia mollis lectin has already established cytokine induction in Th1 and Th2 pathways.
Thereby, this study aimed to evaluate Cramoll 1, 4 in IL-6, IL-17A, IL-22, and IL-23 induction as well as analyze immunologic memory mechanism by reinducing lymphocyte stimulation.
Initially we performed a screening in cultured splenocytes where Cramoll 1, 4 stimulated IL-6 production 5x more than ConA (P<0.05).
The same behavior was observed with IL-22 where the increase was greater than 4x.
Nevertheless, IL-17A induction was similar for both lectins.
In PBMCs, the same splenocytes course was observed for IL-6 and IL-17A.
Concerning the stimulation of IL-22 and IL-23 Cramoll 1, 4 was more efficient than ConA in cytokines stimulation mainly in IL-23 (P<0.01).
Analyzing reinduced lymphocyte stimulation, IL-17A production was higher (P<0.001) when the first stimulus was realized with Cramoll 1, 4 at 1 μg/mL and the second at 5 μg/mL.
IL-22 shows significant differences (P<0.01) at the same condition.
Nevertheless, IL-23 revels the best response when the first stimuli was realized with Cramoll1, 4 at 100 ng/mL and the second with 5 μg/mL.
We conclude that the Cramoll 1, 4 is able to induce IL-6, IL-17A, IL-22, and IL-23 cytokines in vitro better than Concavalin A, besides immunologic memory generation, being a potential biotechnological tool in Th17 pathway studies.
American Psychological Association (APA)
Oliveira, Priscilla Stela Santana de& Rêgo, Moacyr Jesus Barreto de Melo& da Silva, Rafael Ramos& Cavalcanti, Mariana Brayner& Galdino, Suely Lins& Correia, Maria Tereza dos Santos…[et al.]. 2013. Cratylia mollis 1, 4 Lectin: A New Biotechnological Tool in IL-6, IL-17A, IL-22, and IL-23 Induction and Generation of Immunological Memory. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003825
Modern Language Association (MLA)
Oliveira, Priscilla Stela Santana de…[et al.]. Cratylia mollis 1, 4 Lectin: A New Biotechnological Tool in IL-6, IL-17A, IL-22, and IL-23 Induction and Generation of Immunological Memory. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1003825
American Medical Association (AMA)
Oliveira, Priscilla Stela Santana de& Rêgo, Moacyr Jesus Barreto de Melo& da Silva, Rafael Ramos& Cavalcanti, Mariana Brayner& Galdino, Suely Lins& Correia, Maria Tereza dos Santos…[et al.]. Cratylia mollis 1, 4 Lectin: A New Biotechnological Tool in IL-6, IL-17A, IL-22, and IL-23 Induction and Generation of Immunological Memory. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1003825
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003825