A Guide RNA Sequence Design Platform for the CRISPRCas9 System for Model Organism Genomes
Joint Authors
Ma, Ming
Ye, Adam Y.
Zheng, Weiguo
Kong, Lei
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-03
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells.
Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms.
The platform is easy to use for gRNA design with input query sequences.
It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content.
The platform allows users to upload and share their experimental results.
In addition, most guide RNA sequences from published papers have been put into our database.
American Psychological Association (APA)
Ma, Ming& Ye, Adam Y.& Zheng, Weiguo& Kong, Lei. 2013. A Guide RNA Sequence Design Platform for the CRISPRCas9 System for Model Organism Genomes. BioMed Research International،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1003853
Modern Language Association (MLA)
Ma, Ming…[et al.]. A Guide RNA Sequence Design Platform for the CRISPRCas9 System for Model Organism Genomes. BioMed Research International No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-1003853
American Medical Association (AMA)
Ma, Ming& Ye, Adam Y.& Zheng, Weiguo& Kong, Lei. A Guide RNA Sequence Design Platform for the CRISPRCas9 System for Model Organism Genomes. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-1003853
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003853
