NSOMQD-Based Visualization of GM1 Serving as Platforms for TCRCD3 Mediated T-Cell Activation
Joint Authors
Zhong, Liyun
Zhang, Zhun
Lu, Xiaoxu
Liu, Shengde
Chen, Crystal Y.
Chen, Zheng W.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Direct molecular imaging of nanoscale relationship between T-cell receptor complexes (TCR/CD3) and gangliosidosis GM1 before and after T-cell activation has not been reported.
In this study, we made use of our expertise of near-field scanning optical microscopy(NSOM)/immune-labeling quantum dots- (QD-)based dual-color imaging system to visualize nanoscale profiles for distribution and organization of TCR/CD3, GM1, as well as their nanospatial relationship and their correlation with PKCθ signaling cascade during T-cell activation.
Interestingly, after anti-CD3/anti-CD28 Ab co-stimulation, both TCR/CD3 and GM1 were clustered to form nanodomains; moreover, all of TCR/CD3 nanodomains were colocalized with GM1 nanodomains, indicating that the formation of GM1 nanodomains was greatly correlated with TCR/CD3 mediated signaling.
Specially, while T-cells were pretreated with PKCθ signaling inhibitor rottlerin to suppress IL-2 cytokine production, no visible TCR/CD3 nanodomains appeared while a lot of GM1 nanodomains were still observed.
However, while T-cells are pretreated with PKCαβ signaling inhibitor GÖ6976 to suppress calcium-dependent manner, all of TCR/CD3 nanodomains were still colocalized with GM1 nanodomains.
These findings possibly support the notion that the formation of GM1 nanodomains indeed serves as platforms for the recruitment of TCR/CD3 nanodomains, and TCR/CD3 nanodomains are required for PKCθ signaling cascades and T-cell activation
American Psychological Association (APA)
Zhong, Liyun& Zhang, Zhun& Lu, Xiaoxu& Liu, Shengde& Chen, Crystal Y.& Chen, Zheng W.. 2013. NSOMQD-Based Visualization of GM1 Serving as Platforms for TCRCD3 Mediated T-Cell Activation. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003865
Modern Language Association (MLA)
Zhong, Liyun…[et al.]. NSOMQD-Based Visualization of GM1 Serving as Platforms for TCRCD3 Mediated T-Cell Activation. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1003865
American Medical Association (AMA)
Zhong, Liyun& Zhang, Zhun& Lu, Xiaoxu& Liu, Shengde& Chen, Crystal Y.& Chen, Zheng W.. NSOMQD-Based Visualization of GM1 Serving as Platforms for TCRCD3 Mediated T-Cell Activation. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1003865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003865