Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng
Joint Authors
Hu, Wei
Liu, Ning
Tian, Yuhua
Zhang, Lianxue
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The objective of this study is to clone and charecterize the expression of dammarenediol synthase gene and then to determine the relationship between the expression of dammarenediol synthase gene that is involved in the ginsenoside biosynthetic pathway and the ginsenoside content.
A cDNA phage library was constructed from a five-year-old ginseng root.
The cDNA library was screened for the dammarenediol synthase gene by using its specific primers.
It was further cloned and expressed in pET-30a vector.
The recombinant plasmid pET-30a-DS was expressed in Rosetta E.
coli.
The recombinant DS protein was purified by affinity chromatography.
The production of dammarenediol was detected by liquid chromatography-mass spectrometry (LC-MS).
Results showed that dammarenediol synthase gene was cloned from the cDNA library and was expressed in Rosetta E.
coli and the SDS-PAGE analysis showed the presence of purified DS protein.
LS-MS showed the activity of DS protein, as the protein content increases the dammarenediol increases.
Our results indicate that the recombinant dammarenediol synthase protein could increase the production of dammarenediol and the expression of DS played a vital role in the biosynthesis of ginsenosides in P.
ginseng.
American Psychological Association (APA)
Hu, Wei& Liu, Ning& Tian, Yuhua& Zhang, Lianxue. 2012. Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003900
Modern Language Association (MLA)
Hu, Wei…[et al.]. Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1003900
American Medical Association (AMA)
Hu, Wei& Liu, Ning& Tian, Yuhua& Zhang, Lianxue. Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng. BioMed Research International. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1003900
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003900