Digital Microscopy Assessment of Angiogenesis in Different Breast Cancer Compartments
Joint Authors
Haisan, Anca
Rogojanu, Radu
Croitoru, Camelia
Jitaru, Daniela
Tarniceriu, Cristina
Danciu, Mihai
Carasevici, Eugen
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background/Aim.
Tumour angiogenesis defined by microvessel density (MVD) is generally accepted as a prognostic factor in breast cancer.
However, due to variability of measurement systems and cutoffs, it is questionable to date whether it contributes to predictive outline.
Our study aims to grade vascular heterogeneity by comparing clear-cut compartments: tumour associated stroma (TAS), tumour parenchyma, and tumour invasive front.
Material and Methods.
Computerized vessel area measurement was performed using a tissue cytometry system (TissueFAXS) on slides originated from 50 patients with breast cancer.
Vessels were marked using immunohistochemistry with CD34.
Regions of interest were manually defined for each tumour compartment.
Results.
Tumour invasive front vascular endothelia area was 2.15 times higher than that in tumour parenchyma and 4.61 times higher than that in TAS (P<0.002).
Worth to mention that the lymph node negative subgroup of patients show a slight but constant increase of vessel index in all examined compartments of breast tumour.
Conclusion.
Whole slide digital examination and region of interest (ROI) analysis are a valuable tool in scoring angiogenesis markers and disclosing their prognostic capacity.
Our study reveals compartments’ variability of vessel density inside the tumour and highlights the propensity of invasive front to associate an active process of angiogenesis with potential implications in adjuvant therapy.
American Psychological Association (APA)
Haisan, Anca& Rogojanu, Radu& Croitoru, Camelia& Jitaru, Daniela& Tarniceriu, Cristina& Danciu, Mihai…[et al.]. 2013. Digital Microscopy Assessment of Angiogenesis in Different Breast Cancer Compartments. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003902
Modern Language Association (MLA)
Haisan, Anca…[et al.]. Digital Microscopy Assessment of Angiogenesis in Different Breast Cancer Compartments. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1003902
American Medical Association (AMA)
Haisan, Anca& Rogojanu, Radu& Croitoru, Camelia& Jitaru, Daniela& Tarniceriu, Cristina& Danciu, Mihai…[et al.]. Digital Microscopy Assessment of Angiogenesis in Different Breast Cancer Compartments. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1003902
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003902