Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses
Joint Authors
Foord, Adam J.
White, John R.
Colling, Axel
Heine, Hans G.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Microsphere suspension array systems enable the simultaneous fluorescent identification of multiple separate nucleotide targets in a single reaction.
We have utilized commercially available oligo-tagged microspheres (Luminex MagPlex-TAG) to construct and evaluate multiplexed assays for the detection and differentiation of Hendra virus (HeV) and Nipah virus (NiV).
Both these agents are bat-borne zoonotic paramyxoviruses of increasing concern for veterinary and human health.
Assays were developed targeting multiple sites within the nucleoprotein (N) and phosphoprotein (P) encoding genes.
The relative specificities and sensitivities of the assays were determined using reference isolates of each virus type, samples from experimentally infected horses, and archival veterinary diagnostic submissions.
Results were assessed in direct comparison with an established qPCR.
The microsphere array assays achieved unequivocal differentiation of HeV and NiV and the sensitivity of HeV detection was comparable to qPCR, indicating high analytical and diagnostic specificity and sensitivity.
American Psychological Association (APA)
Foord, Adam J.& White, John R.& Colling, Axel& Heine, Hans G.. 2013. Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003908
Modern Language Association (MLA)
Foord, Adam J.…[et al.]. Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003908
American Medical Association (AMA)
Foord, Adam J.& White, John R.& Colling, Axel& Heine, Hans G.. Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003908