Membrane Localization of Membrane Type 1 Matrix Metalloproteinase by CD44 Regulates the Activation of Pro-Matrix Metalloproteinase 9 in Osteoclasts
Joint Authors
Chellaiah, Meenakshi A.
Ma, Tao
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
CD44, MT1-MMP, and MMP9 are implicated in the migration of osteoclast and bone resorption.
This study was designed to determine the functional relationship between CD44 and MT1-MMP in the activation of pro-MMP9.
We used osteoclasts isolated from wild-type and CD44-null mice.
Results showed that MT1-MMP is present in multiple forms with a molecular mass ~63, 55, and 45 kDa in the membrane of wild-type osteoclasts.
CD44-null osteoclasts demonstrated a 55 kDa active MT1-MMP form in the membrane and conditioned medium.
It failed to activate pro-MMP9 because TIMP2 binds and inhibits this MT1-MMP (~55 kDa) in CD44-null osteoclasts.
The role of MT1-MMP in the activation of pro-MMP9, CD44 expression, and migration was confirmed by knockdown of MT1-MMP in wild-type osteoclasts.
Although knockdown of MMP9 suppressed osteoclast migration, it had no effects on MT1-MMP activity or CD44 expression.
These results suggest that CD44 and MT1-MMP are directly or indirectly involved in the regulation of pro-MMP9 activation.
Surface expression of CD44, membrane localization of MT1-MMP, and activation of pro-MMP9 are the necessary sequence of events in osteoclast migration.
American Psychological Association (APA)
Chellaiah, Meenakshi A.& Ma, Tao. 2013. Membrane Localization of Membrane Type 1 Matrix Metalloproteinase by CD44 Regulates the Activation of Pro-Matrix Metalloproteinase 9 in Osteoclasts. BioMed Research International،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1003947
Modern Language Association (MLA)
Chellaiah, Meenakshi A.& Ma, Tao. Membrane Localization of Membrane Type 1 Matrix Metalloproteinase by CD44 Regulates the Activation of Pro-Matrix Metalloproteinase 9 in Osteoclasts. BioMed Research International No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1003947
American Medical Association (AMA)
Chellaiah, Meenakshi A.& Ma, Tao. Membrane Localization of Membrane Type 1 Matrix Metalloproteinase by CD44 Regulates the Activation of Pro-Matrix Metalloproteinase 9 in Osteoclasts. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1003947
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003947