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Assessing the Detection Capacity of Microarrays as BioNanosensing Platforms
Joint Authors
Lee, Ju Seok
Song, Joon Jin
Deaton, Russell
Kim, Jin-Woo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Microarray is one of the most powerful detection systems with multiplexing and high throughput capability.
It has significant potential as a versatile biosensing platform for environmental monitoring, pathogen detection, medical therapeutics, and drug screening to name a few.
To date, however, microarray applications are still limited to preliminary screening of genome-scale transcription profiling or gene ontology analysis.
Expanding the utility of microarrays as a detection tool for various biological and biomedical applications requires information about performance such as the limits of detection and quantification, which are considered as an essential information to decide the detection sensitivity of sensing devices.
Here we present a calibration design that integrates detection limit theory and linear dynamic range to obtain a performance index of microarray detection platform using oligonucleotide arrays as a model system.
Two different types of limits of detection and quantification are proposed by the prediction or tolerance interval for two common cyanine fluorescence dyes, Cy3 and Cy5.
Besides oligonucleotide, the proposed method can be generalized to other microarray formats with various biomolecules such as complementary DNA, protein, peptide, carbohydrate, tissue, or other small biomolecules.
Also, it can be easily applied to other fluorescence dyes for further dye chemistry improvement.
American Psychological Association (APA)
Lee, Ju Seok& Song, Joon Jin& Deaton, Russell& Kim, Jin-Woo. 2013. Assessing the Detection Capacity of Microarrays as BioNanosensing Platforms. BioMed Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003981
Modern Language Association (MLA)
Lee, Ju Seok…[et al.]. Assessing the Detection Capacity of Microarrays as BioNanosensing Platforms. BioMed Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1003981
American Medical Association (AMA)
Lee, Ju Seok& Song, Joon Jin& Deaton, Russell& Kim, Jin-Woo. Assessing the Detection Capacity of Microarrays as BioNanosensing Platforms. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1003981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003981