Preparative Purification of Recombinant Proteins: Current Status and Future Trends
Joint Authors
Saraswat, Mayank
Musante, Luca
Ravidá, Alessandra
Shortt, Brian
Byrne, Barry
Holthofer, Harry
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Advances in fermentation technologies have resulted in the production of increased yields of proteins of economic, biopharmaceutical, and medicinal importance.
Consequently, there is an absolute requirement for the development of rapid, cost-effective methodologies which facilitate the purification of such products in the absence of contaminants, such as superfluous proteins and endotoxins.
Here, we provide a comprehensive overview of a selection of key purification methodologies currently being applied in both academic and industrial settings and discuss how innovative and effective protocols such as aqueous two-phase partitioning, membrane chromatography, and high-performance tangential flow filtration may be applied independently of or in conjunction with more traditional protocols for downstream processing applications.
American Psychological Association (APA)
Saraswat, Mayank& Musante, Luca& Ravidá, Alessandra& Shortt, Brian& Byrne, Barry& Holthofer, Harry. 2013. Preparative Purification of Recombinant Proteins: Current Status and Future Trends. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1003988
Modern Language Association (MLA)
Saraswat, Mayank…[et al.]. Preparative Purification of Recombinant Proteins: Current Status and Future Trends. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1003988
American Medical Association (AMA)
Saraswat, Mayank& Musante, Luca& Ravidá, Alessandra& Shortt, Brian& Byrne, Barry& Holthofer, Harry. Preparative Purification of Recombinant Proteins: Current Status and Future Trends. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1003988
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1003988