Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism
Joint Authors
Li, Wei
Hu, Zhen-Fu
Chen, Bin
Ni, Guo-Xin
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Oxygen and glucose are two important nutrients for mammalian cell function.
In this study, the effect of glucose and oxygen concentrations on C2C12 cellular metabolism was characterized with an emphasis on detecting whether cells show oxygen conformance (OC) in response to hypoxia.
Methods.
After C2C12 cells being cultured in the levels of glucose at 0.6 mM (LG), 5.6 mM (MG), or 23.3 mM(HG) under normoxic or hypoxic (1% oxygen) condition, cellular oxygen consumption, glucose consumption, lactate production, and metabolic status were determined.
Short-term oxygen consumption was measured with a novel oxygen biosensor technique.
Longer-term measurements were performed with standard glucose, lactate, and cell metabolism assays.
Results.
It was found that oxygen depletion in normoxia is dependent on the glucose concentration in the medium.
Cellular glucose uptake and lactate production increased significantly in hypoxia than those in normoxia.
In hypoxia the cellular response to the level of glucose was different to that in normoxia.
The metabolic activities decreased while glucose concentration increased in normoxia, while in hypoxia, metabolic activity was reduced in LG and MG, but unchanged in HG condition.
The OC phenomenon was not observed in the present study.
Conclusions.
Our findings suggested that a combination of low oxygen and low glucose damages the viability of C2C12 cells more seriously than low oxygen alone.
In addition, when there is sufficient glucose, C2C12 cells will respond to hypoxia by upregulating anaerobic respiration, as shown by lactate production.
American Psychological Association (APA)
Li, Wei& Hu, Zhen-Fu& Chen, Bin& Ni, Guo-Xin. 2013. Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004030
Modern Language Association (MLA)
Li, Wei…[et al.]. Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1004030
American Medical Association (AMA)
Li, Wei& Hu, Zhen-Fu& Chen, Bin& Ni, Guo-Xin. Response of C2C12 Myoblasts to Hypoxia: The Relative Roles of Glucose and Oxygen in Adaptive Cellular Metabolism. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004030
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004030