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High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production in Escherichia coli
Joint Authors
Sankar, S. Gowri
Dhanajeyan, K. J.
Paramasivan, R.
Thenmozhi, V.
Tyagi, B. K.
Vennison, S. John
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Detection of nonstructural protein (NS1) is an important diagnostic marker during acute phase of dengue infection.
Not only for diagnostic purpose, the protein had important role in vaccine design as well, as a candidate for studying virus assembly and maturation.
Various researchers employed different expression systems and strategies for recombinant NS1 protein production.
Attempts to express NS1 protein in prokaryotic and yeast expression system result in formation of insoluble protein which needs to undergo refolding to attain native structural and functional forms.
Here, we report the production of soluble NS1 protein in E.
coli by using appropriate vector and employing suitable culture conditions to maximize protein production.
Proteins were purified using metal affinity chromatography.
SDS-PAGE and western blot analysis reveal the native structure of NS1 protein.
Solid phase ELISA using the recombinantly expressed antigen with positive and negative dengue samples showed that the expressed protein retains its antigenic and immunological properties.
To our knowledge, this is the first report on the successful production of functionally active recombinant dengue-2 NS1 protein production without undergoing any in vitro posttranslational modification process.
American Psychological Association (APA)
Sankar, S. Gowri& Dhanajeyan, K. J.& Paramasivan, R.& Thenmozhi, V.& Tyagi, B. K.& Vennison, S. John. 2013. High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production in Escherichia coli. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004056
Modern Language Association (MLA)
Sankar, S. Gowri…[et al.]. High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production in Escherichia coli. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1004056
American Medical Association (AMA)
Sankar, S. Gowri& Dhanajeyan, K. J.& Paramasivan, R.& Thenmozhi, V.& Tyagi, B. K.& Vennison, S. John. High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production in Escherichia coli. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004056