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Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction
Joint Authors
Dominici, Luca
Guerrera, Elena
Villarini, Milena
Fatigoni, Cristina
Moretti, Massimo
Blasi, Paolo
Monarca, Silvano
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In tunnel construction, workers exposed to dust from blasting, gases, diesel exhausts, and oil mist have shown higher risk for pulmonary diseases.
A clear mechanism to explain how these pollutants determine diseases is lacking, and alveolar epithelium’s capacity to ingest inhaled fine particles is not well characterized.
The objective of this study was to assess the genotoxic effect exerted by fine particles collected in seven tunnels using the cytokinesis-block micronuclei test in an in vitro model on type II lung epithelium A549 cells.
For each tunnel, five fractions with different aerodynamic diameters of particulate matter were collected with a multistage cascade sampler.
The human epithelial cell line A549 was exposed to 0.2 m3/mL equivalent of particulate for 24 h before testing.
The cytotoxic effects of particulate matter on A549 cells were also evaluated in two different viability tests.
In order to evaluate the cells’ ability to take up fine particles, imaging with transmission electron microscopy of cells after exposure to particulate matter was performed.
Particle endocytosis after 24 h exposure was observed as intracellular aggregates of membrane-bound particles.
This morphologic evidence did not correspond to an increase in genotoxicity detected by the micronucleus test.
American Psychological Association (APA)
Dominici, Luca& Guerrera, Elena& Villarini, Milena& Fatigoni, Cristina& Moretti, Massimo& Blasi, Paolo…[et al.]. 2013. Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004061
Modern Language Association (MLA)
Dominici, Luca…[et al.]. Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004061
American Medical Association (AMA)
Dominici, Luca& Guerrera, Elena& Villarini, Milena& Fatigoni, Cristina& Moretti, Massimo& Blasi, Paolo…[et al.]. Evaluation of In Vitro Cytoxicity and Genotoxicity of Size-Fractionated Air Particles Sampled during Road Tunnel Construction. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004061
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004061