Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
Joint Authors
Pratumvinit, Busadee
Reesukumal, Kanit
Janebodin, Kajohnkiart
Ieronimakis, Nicholas
Reyes, Morayma
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-27
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Isolation and ex vivo expansion of cardiac endothelial cells have been a recurrent challenge due to difficulties in isolation, cell heterogeneity, lack of specific markers to identify myocardial endothelial cells, and inadequate conditions to maintain long-term cultures.
Herein, we developed a method for isolation, characterization, and expansion of cardiac endothelial cells applicable to study endothelial cell biology and clinical applications such as neoangiogenesis.
First, we dissociated the cells from murine heart by mechanical disaggregation and enzymatic digestion.
Then, we used flow cytometry coupled with specific markers to isolate endothelial cells from murine hearts.
CD45+ cells were gated out to eliminate the hematopoietic cells.
CD31+/Sca-1+ cells were isolated as endothelial cells.
Cells isolated from atrium grew faster than those from ventricle.
Cardiac endothelial cells maintain endothelial cell function such as vascular tube formation and acetylated-LDL uptake in vitro.
Finally, cardiac endothelial cells formed microvessels in dorsal matrigel plug and engrafted in cardiac microvessels following intravenous and intra-arterial injections.
In conclusion, our multicolor flow cytometry method is an effective method to analyze and purify endothelial cells from murine heart, which in turn can be ex vivo expanded to study the biology of endothelial cells or for clinical applications such as therapeutic angiogenesis.
American Psychological Association (APA)
Pratumvinit, Busadee& Reesukumal, Kanit& Janebodin, Kajohnkiart& Ieronimakis, Nicholas& Reyes, Morayma. 2013. Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004107
Modern Language Association (MLA)
Pratumvinit, Busadee…[et al.]. Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. BioMed Research International No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-1004107
American Medical Association (AMA)
Pratumvinit, Busadee& Reesukumal, Kanit& Janebodin, Kajohnkiart& Ieronimakis, Nicholas& Reyes, Morayma. Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-1004107
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004107