Identification, Selection, and Enrichment of Cardiomyocyte Precursors
Joint Authors
Zanetti, Bianca Ferrarini
Gomes, Walter José
Han, Sang Won
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia.
The main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mesenchymal stem cells from adipose tissue.
First, lentiviral vectors expressing neoR and GFP under the control of promoters expressed specifically during cardiomyogenesis were constructed to monitor cell reprogramming into precardiomyocytes and to select cells for amplification and characterization.
Cellular reprogramming was performed using 5′-azacytidine followed by electroporation with plasmid pOKS2a, which expressed Oct4, Sox2, and Klf4.
Under these conditions, GFP expression began only after transfection with pOKS2a, and less than 0.015% of cells were GFP+.
These GFP+ cells were selected for G418 resistance to find molecular markers of cardiomyocytes by RT-PCR and immunocytochemistry.
Both genetic and protein markers of cardiomyocytes were present in the selected cells, with some variations among them.
Cell doubling time did not change after selection.
Together, these results indicate that enrichment with vectors expressing GFP and neoR under cardiomyocyte-specific promoters can produce large numbers of cardiomyocyte precursors (CMPs), which can then be differentiated terminally for cell therapy and tissue engineering.
American Psychological Association (APA)
Zanetti, Bianca Ferrarini& Gomes, Walter José& Han, Sang Won. 2013. Identification, Selection, and Enrichment of Cardiomyocyte Precursors. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004182
Modern Language Association (MLA)
Zanetti, Bianca Ferrarini…[et al.]. Identification, Selection, and Enrichment of Cardiomyocyte Precursors. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004182
American Medical Association (AMA)
Zanetti, Bianca Ferrarini& Gomes, Walter José& Han, Sang Won. Identification, Selection, and Enrichment of Cardiomyocyte Precursors. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004182
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004182