Acid Lipase from Candida viswanathii: Production, Biochemical Properties, and Potential Application

Abstract EN

Influences of environmental variables and emulsifiers on lipase production of a Candida viswanathii strain were investigated.

The highest lipase activity (101.1 U) was observed at 210 rpm, pH 6.0, and 27.5°C.

Other fermentation parameters analyzed showed considerable rates of biomass yield (YL/S=1.381 g/g), lipase yield (YL/S=6.892 U/g), and biomass productivity (PX=0.282 g/h).

Addition of soybean lecithin increased lipase production in 1.45-fold, presenting lipase yield (YL/S) of 10.061 U/g.

Crude lipase presented optimal activity at acid pH of 3.5, suggesting a new lipolytic enzyme for this genus and yeast in general.

In addition, crude lipase presented high stability in acid conditions and temperature between 40 and 45°C, after 24 h of incubation in these temperatures.

Lipase remained active in the presence of organic solvents maintaining above 80% activity in DMSO, methanol, acetonitrile, ethanol, acetone, 1-propanol, isopropanol, and 2-propanol.

Effectiveness for the hydrolysis of a wide range of natural triglycerides suggests that this new acid lipase has high potential application in the oleochemical and food industries for hydrolysis and/or modification of triacylglycerols to improve the nutritional properties.