Chromogenic In Situ Hybridization and p16Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6E7 mRNA Test, and Potential Clinical Applications
Joint Authors
Zappacosta, Roberta
Colasante, Antonella
Viola, Patrizia
D’Antuono, Tommaso
Lattanzio, Giuseppe
Capanna, Serena
Gatta, Daniela Maria Pia
Rosini, Sandra
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Although HPV-DNA test and E6/E7 mRNA analyses remain the current standard for the confirmation of human papillomavirus (HPV) infections in cytological specimens, no universally adopted techniques exist for the detection of HPV in formalin-fixed paraffin-embedded samples.
Particularly, in routine laboratories, molecular assays are still time-consuming and would require a high level of expertise.
In this study, we investigated the possible use of a novel HPV tyramide-based chromogenic in situ hybridization (CISH) technology to locate HPV on tissue specimens.
Then, we evaluate the potential usefulness of p16INK4a/Ki-67 double stain on histological samples, to identify cervical cells expressing HPV E6/E7 oncogenes.
In our series, CISH showed a clear signal in 95.2% of the specimens and reached a sensitivity of 86.5%.
CISH positivity always matched with HPV-DNA positivity, while 100% of cases with punctated signal joined with cervical intraepithelial neoplasia grade 2 or worse (CIN2+).
p16/Ki67 immunohistochemistry gave an interpretable result in 100% of the cases.
The use of dual stain significantly increased the agreement between pathologists, which reached 100%.
Concordance between dual stain and E6/E7 mRNA test was 89%.
In our series, both CISH and p16INK4a/Ki67 dual stain demonstrated high grade of performances.
In particular, CISH would help to distinguish episomal from integrated HPV, in order to allow conclusions regarding the prognosis of the lesion, while p16INK4a/Ki67 dual stain approach would confer a high level of standardization to the diagnostic procedure.
American Psychological Association (APA)
Zappacosta, Roberta& Colasante, Antonella& Viola, Patrizia& D’Antuono, Tommaso& Lattanzio, Giuseppe& Capanna, Serena…[et al.]. 2013. Chromogenic In Situ Hybridization and p16Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6E7 mRNA Test, and Potential Clinical Applications. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004320
Modern Language Association (MLA)
Zappacosta, Roberta…[et al.]. Chromogenic In Situ Hybridization and p16Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6E7 mRNA Test, and Potential Clinical Applications. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004320
American Medical Association (AMA)
Zappacosta, Roberta& Colasante, Antonella& Viola, Patrizia& D’Antuono, Tommaso& Lattanzio, Giuseppe& Capanna, Serena…[et al.]. Chromogenic In Situ Hybridization and p16Ki67 Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6E7 mRNA Test, and Potential Clinical Applications. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004320