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Transcriptional Response of Bovine Monocyte-Derived Macrophages after the Infection with Different Argentinean Mycobacterium bovis Isolates
Joint Authors
Blanco, Federico Carlos
Caimi, Karina
Bigi, Fabiana
Soria, Marcelo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Infection of bovines with Mycobacterium bovis causes important financial hardship in many countries presenting also a risk for humans.
M.
bovis is known to be adapted to survive and thrive within the intramacrophage environment.
In spite of its relevance, at present the information about macrophage expression patterns is scarce, particularly regarding the bovine host.
In this study, transcriptomic analysis was used to detect genes differentially expressed in macrophages derived from peripheral blood mononuclear cells at early stages of infection with two Argentinean strains of M.
bovis, a virulent and an attenuated strains.
The results showed that the number of differentially expressed genes in the cells infected with the virulent strain (5) was significantly lower than those in the cells infected with the attenuated strain (172).
Several genes were more strongly expressed in infected macrophages.
Among them, we detected encoding transcription factors, anthrax toxin receptor, cell division and apoptosis regulator, ankyrin proteins, cytoskeleton proteins, protein of cell differentiation, and regulators of endocytic traffic of membrane.
Quantitative real-time PCR of a selected group of differentially expressed genes confirmed the microarrays results.
Altogether, the present results contribute to understanding the mechanisms involved in the early interaction of M.
bovis with the bovine macrophage.
American Psychological Association (APA)
Caimi, Karina& Blanco, Federico Carlos& Soria, Marcelo& Bigi, Fabiana. 2013. Transcriptional Response of Bovine Monocyte-Derived Macrophages after the Infection with Different Argentinean Mycobacterium bovis Isolates. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004326
Modern Language Association (MLA)
Caimi, Karina…[et al.]. Transcriptional Response of Bovine Monocyte-Derived Macrophages after the Infection with Different Argentinean Mycobacterium bovis Isolates. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004326
American Medical Association (AMA)
Caimi, Karina& Blanco, Federico Carlos& Soria, Marcelo& Bigi, Fabiana. Transcriptional Response of Bovine Monocyte-Derived Macrophages after the Infection with Different Argentinean Mycobacterium bovis Isolates. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004326
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004326