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Improvement of Daptomycin Production in Streptomyces roseosporus through the Acquisition of Pleuromutilin Resistance
Joint Authors
Li, Linli
Ma, Tingmei
Liu, Qing
Huang, Yuqi
Hu, Changhua
Liao, Guojian
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Daptomycin, a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, displays potent activity against a variety of gram-positive pathogens.
There is a demand for generating high-producing strains for industrial production of this valuable antibiotic.
Ribosome engineering is a powerful strategy to enhance the yield of secondary metabolites.
In this study, the effect of a diterpenoid antibiotic pleuromutilin resistance mutation on daptomycin production was assessed.
Spontaneous pleuromutilin-resistant derivatives of S.
roseosporus were isolated.
Sequencing of rplC locus (encoding the ribosomal protein L3) showed a point mutation at nt 455, resulting in the substitution of glycine with valine.
G152V mutants showed increased production of daptomycin by approximately 30% in comparison with the wild-type strain.
Its effect on daptomycin production was due to enhanced gene transcription of the daptomycin biosynthetic genes.
In conclusion, pleuromutilin could be used as a novel ribosome engineering agent to improve the production of desired secondary metabolites.
American Psychological Association (APA)
Li, Linli& Ma, Tingmei& Liu, Qing& Huang, Yuqi& Hu, Changhua& Liao, Guojian. 2013. Improvement of Daptomycin Production in Streptomyces roseosporus through the Acquisition of Pleuromutilin Resistance. BioMed Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004403
Modern Language Association (MLA)
Li, Linli…[et al.]. Improvement of Daptomycin Production in Streptomyces roseosporus through the Acquisition of Pleuromutilin Resistance. BioMed Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1004403
American Medical Association (AMA)
Li, Linli& Ma, Tingmei& Liu, Qing& Huang, Yuqi& Hu, Changhua& Liao, Guojian. Improvement of Daptomycin Production in Streptomyces roseosporus through the Acquisition of Pleuromutilin Resistance. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1004403
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004403