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DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals
Author
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Exposure of cells and organisms to stressors might result in epigenetic changes.
Here it is shown that investigation of DNA methylation using pyrosequencing is an alternative for in vitro and in vivo toxicological testing of epigenetic effects induced by chemicals and drugs.
An in vitro evaluation of global and CpG site specific DNA methylation upon treatment of cells with chemicals/drugs is shown.
Bisulfite genomic sequencing of methylation controls showed high methylation of LINE1 in methylation positive control and low methylation in the negative controls.
The CpG sites within the LINE1 element are methylated at different levels.
In vitro cell cultures show a methylation level ranging from 56% to 49%.
Cultures of drug resistant tumor cells show significant hypomethylation as compared with the originating nonresistant tumor cells.
The in vitro testing of epigenetically active chemicals (5-methyl-2’-deoxycytidine and trichostatin A) revealed a significant change of LINE1 methylation status upon treatment, while specific CpG sites were more prone to demethylation than others (focal methylation).
In conclusion, DNA methylation using pyrosequencing might be used not only for testing epigenetic toxins/drugs but also in risk assessment of drugs, food, and environmental relevant pollutants.
American Psychological Association (APA)
Florea, Ana-Maria. 2013. DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals. BioMed Research International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004430
Modern Language Association (MLA)
Florea, Ana-Maria. DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals. BioMed Research International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1004430
American Medical Association (AMA)
Florea, Ana-Maria. DNA Methylation Pyrosequencing Assay Is Applicable for the Assessment of Epigenetic Active Environmental or Clinical Relevant Chemicals. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1004430
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004430