Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi
Joint Authors
Lugo-Caballero, C.
Manning-Cela, Rebeca G.
Ballesteros-Rodea, G.
Martínez-Calvillo, Santiago
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To carry out the intracellular phase of its life cycle, Trypanosoma cruzi must infect a host cell.
Although a few molecules have been reported to participate in this process, one known protein is LYT1, which promotes lysis under acidic conditions and is involved in parasite infection and development.
Alternative transcripts from a single LYT1 gene generate two proteins with differential functions and compartmentalization.
Single-gene products targeted to more than one location can interact with disparate proteins that might affect their function and targeting properties.
The aim of this work was to study the LYT1 interaction map using coimmunoprecipitation assays with transgenic parasites expressing LYT1 products fused to GFP.
We detected several proteins of sizes from 8 to 150 kDa that bind to LYT1 with different binding strengths.
By MS-MS analysis, we identified proteins involved in parasite infectivity (trans-sialidase), development (kDSPs and histones H2A and H2B), and motility and protein traffic (dynein and α- and β-tubulin), as well as protein-protein interactions (TPR-protein and kDSPs) and several hypothetical proteins.
Our approach led us to identify the LYT1 interaction profile, thereby providing insights into the molecular mechanisms that contribute to parasite stage development and pathogenesis of T.
cruzi infection.
American Psychological Association (APA)
Lugo-Caballero, C.& Ballesteros-Rodea, G.& Martínez-Calvillo, Santiago& Manning-Cela, Rebeca G.. 2013. Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi. BioMed Research International،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004448
Modern Language Association (MLA)
Lugo-Caballero, C.…[et al.]. Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi. BioMed Research International No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1004448
American Medical Association (AMA)
Lugo-Caballero, C.& Ballesteros-Rodea, G.& Martínez-Calvillo, Santiago& Manning-Cela, Rebeca G.. Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1004448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004448