Effect of CN Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611

Abstract EN

The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611.

From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective.

Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition.

The effect of five main reaction parameters including sucrose, yeast extract, NaNO3, Zn+2, and Triton X-100 on the production of enzymes was analyzed.

A modified quadratic model was fitted to the data with a coefficient of determination (R2) more than 0.90 for all responses.

The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO3, 1.5 mM (v/v) Zn+2, and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively.

The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry.