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Inorganic Phosphate Modulates the Expression of the NaPi-2a Transporter in the trans-Golgi Network and the Interaction with PIST in the Proximal Tubule
Joint Authors
Lanaspa, Miguel A.
Caldas, Yupanqui A.
Breusegem, Sophia Y.
Andrés-Hernando, Ana
Cicerchi, Christina
Levi, Moshe
Sorribas, Victor
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Inorganic phosphate (Pi) homeostasis is maintained by the tight regulation of renal Pi excretion versus reabsorption rates that are in turn modulated by adjusting the number of Pi transporters (mainly NaPi-2a) in the proximal tubules.
In response to some hormones and a high dietary Pi content, NaPi-2a is endocytosed and degraded in the lysosomes; however, we show here that some NaPi-2a molecules are targeted to the trans-Golgi network (TGN) during the endocytosis.
In the TGN, NaPi-2a interacts with PIST (PDZ-domain protein interacting specifically with TC10), a TGN-resident PDZ-domain-containing protein.
The extension of the interaction is proportional to the expression of NaPi-2a in the TGN, and, consistent with that, it is increased with a high Pi diet.
When overexpressed in opossum kidney (OK) cells, PIST retains NaPi-2a in the TGN and inhibits Na-dependent Pi transport.
Overexpression of PIST also prevents the adaptation of OK cells to a low Pi culture medium.
Our data supports the view that NaPi-2a is subjected to retrograde trafficking from the plasma membrane to the TGN using one of the machineries involved in endosomal transport and explains the reported expression of NaPi-2a in the TGN.
American Psychological Association (APA)
Lanaspa, Miguel A.& Caldas, Yupanqui A.& Breusegem, Sophia Y.& Andrés-Hernando, Ana& Cicerchi, Christina& Levi, Moshe…[et al.]. 2013. Inorganic Phosphate Modulates the Expression of the NaPi-2a Transporter in the trans-Golgi Network and the Interaction with PIST in the Proximal Tubule. BioMed Research International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004491
Modern Language Association (MLA)
Lanaspa, Miguel A.…[et al.]. Inorganic Phosphate Modulates the Expression of the NaPi-2a Transporter in the trans-Golgi Network and the Interaction with PIST in the Proximal Tubule. BioMed Research International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1004491
American Medical Association (AMA)
Lanaspa, Miguel A.& Caldas, Yupanqui A.& Breusegem, Sophia Y.& Andrés-Hernando, Ana& Cicerchi, Christina& Levi, Moshe…[et al.]. Inorganic Phosphate Modulates the Expression of the NaPi-2a Transporter in the trans-Golgi Network and the Interaction with PIST in the Proximal Tubule. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1004491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004491