Dose- and Time-Dependent Apoptosis Induced by Avian H9N2 Influenza Virus in Human Cells
Joint Authors
Shahsavandi, Shahla
Ebrahimi, Mohammad Majid
Sadeghi, Kaveh
Mosavi, Seyedeh Zahra
Mohammadi, Ashraf
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
To understand human response to avian H9N2 influenza, we investigated the effects of the viral infection on A549, HepG2, and HeLa cells at low and high MOIs.
To identify virus-host interplay, expression of Mx and NP genes was measured in the cells supernatants.
Cell viability and apoptosis were evaluated by MTT assay, DNA fragmentation, and florescent staining.
The virus titration and NP gene transcript levels indicate lower susceptibility of HeLa cell to H9N2 replication than other cells.
Although H9N2 did produce a faster CPE in HepG2, high dose of the virus induced apoptosis within early stage of A549 infection.
The DNA laddering was enhanced in the cell correlated with increase in virus transcripts.
The undetectable to different regulation levels of Mx gene were observed in response to H9N2 infection suggesting that an insufficient antiviral defense in the noncompetent-IFN HepG2 cell promotes efficient viral replication.
These results showed that the permissivity of HepG2 for H9N2 is comparable with A549; however, liver cells are not target tissue respond to the infection.
These data revealed that the H9N2 virus induced apoptosis signaling via mitochondrial pathway in human alveolar epithelial cells, indicating that the induction may be associated with a dose-dependent manner.
American Psychological Association (APA)
Shahsavandi, Shahla& Ebrahimi, Mohammad Majid& Sadeghi, Kaveh& Mosavi, Seyedeh Zahra& Mohammadi, Ashraf. 2013. Dose- and Time-Dependent Apoptosis Induced by Avian H9N2 Influenza Virus in Human Cells. BioMed Research International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004509
Modern Language Association (MLA)
Shahsavandi, Shahla…[et al.]. Dose- and Time-Dependent Apoptosis Induced by Avian H9N2 Influenza Virus in Human Cells. BioMed Research International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-1004509
American Medical Association (AMA)
Shahsavandi, Shahla& Ebrahimi, Mohammad Majid& Sadeghi, Kaveh& Mosavi, Seyedeh Zahra& Mohammadi, Ashraf. Dose- and Time-Dependent Apoptosis Induced by Avian H9N2 Influenza Virus in Human Cells. BioMed Research International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-1004509
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1004509